生物学杂志

生物学杂志 ›› 2022, Vol. 39 ›› Issue (1): 123-.doi: 10. 3969/ j. issn. 2095-1736. 2022. 01. 123

• 教学研究 • 上一篇    下一篇

T 载体表达蛋白的分子生物学综合实验设计

  

  1. 江苏第二师范学院生命科学与化学化工学院, 南京211222
  • 出版日期:2022-02-18 发布日期:2022-02-15
  • 作者简介:戴谷,博士,副教授,研究方向为细胞及分子生物学,E-mail:gu_dai@sina.com
  • 基金资助:
    江苏第二师范学院教学改革研究课题项目(JSSNUJXGG2017YB09)

T vector for protein expression in molecular biology comprehensive experiments design

  1. School of Life Sciences and Chemical Engineering, Jiangsu Second Normal University, Nanjing 211222, China
  • Online:2022-02-18 Published:2022-02-15

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摘要： 为弥补传统分子生物学实验中α-互补法筛选重组子以及蛋白质的原核诱导表达两项实验相对独立、缺乏连续的不足,探索通过设计基因克隆和蛋白表达一体化的综合性实验,以强化学生对阅读框的认识。分子生物学实验中的综合性实验设计拓展了实验教学的广度与深度,为进一步提升学生的知识、能力和素养奠定基础。

关键词: 分子生物学, 实验教学, T 载体, 载体构建, 蛋白表达

Abstract: In the traditional molecular biology experiment, α-complementation method for screening recombinants and protein expression in prokaryotic cells were two independent experimental technologies. In order to make up for the lack of continuity of these two experiments,a comprehensive experiment of gene cloning and protein expression integrative in one vector was designed to strengthen the students􀆶 understanding of the reading frame. The comprehensive experimental design in molecular biology experiments could expand the span and depth of experimental teaching, and laid the foundation for further promoting students􀆶 knowledge, ability and professionalism.

Key words: molecular biology, experimental teaching, T vector, vector construction, protein expression

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